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Shiladitya DasSarma
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, United States of America

Faculty Member: Microbiology > Microbial Evolution & Genomics [ since 4 July 2001 ]
[ Biography ] [ Homepage ] [ Evaluations ]
Biography

Shiladitya DasSarma is a Professor in the University of Maryland Biotechnology Institute, Center of Marine Biotechnology, and the Graduate Program in Life Sciences, University of Maryland at Baltimore. He received his B.S.(Honors) degree in 1979 in Chemistry from Indiana University and his Ph.D. degree in Biochemistry in 1984 from the Department of Biology at the Massaschusetts Institute of Technology, where he was a student of Nobel Laureate H. Gobind Khorana and Uttam L. RajBhandary. He was a postdoctoral student from 1984-1986 in the Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School with Howard M. Goodman. He served on the faculty of the Department of Microbiology, University of Massachusetts-Amherst from 1986-2001.

Dr. DasSarma's research program is focused on the post-genomics, genomics, biotechnology, and molecular biology of halophilic archaea, and more generally on extremophiles and diverse microorganisms inhabiting our planet. Some specific areas of past and current interest include transposable elements causing genetic variability, DNA rearrangements in chromosome and plasmids, genome mapping and sequencing, structure, transcription, and regulation of photosynthetic and buoyancy genes, gene regulation by oxygen and light, left-handed Z-DNA, DNA replication, and computer applications in molecular biology and education. Dr. DasSarma has co-authored about 100 scientific publications and is co-inventor on three US patents.

Some recent professional activities of Dr. DasSarma include serving as Editor-in-Chief of the BioMed Central online Open Access journal, Saline Systems, and editing the Cold Spring Harbor Laboratory Press manual "Archaea: A Laboratory Manual-Halophiles". He has also served on a variety of National Science Foundation Advisory Panels, as Visiting Program Director of NSF's Metabolic Biochemistry Program, and on a National Institutes of Health Study Section. He co-taught the UMBI-COMB Workshop on Extremophiles and Archaea, and Applied Bioinformatics in the Graduate Program in Life Sciences at UMB. He organized and led the Halobacterium Genome Consortium and participated in the American Academy of Microbiology Colloquium on Microbial Genome Sequencing.

Home page

http://www.umbi.umd.edu/~dassarma

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Evaluations

RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex.
Hale CR, Zhao P, …, Terns RM, Terns MP
Cell 2009 Nov 25 139(5):945-56 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Melinda Capes and Shiladitya DasSarma
Evaluated 10 Mar 2010

A single-base resolution map of an archaeal transcriptome.
Wurtzel O, Sapra R, …, Simmons BA, Sorek R
Genome Res 2010 Jan 20(1):133-41 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | James A Coker and Shiladitya DasSarma
Evaluated 3 Mar 2010

RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro.
Grohmann D, Hirtreiter A, Werner F
Biochem J 2009 Aug 1 421(3):339-43 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | James A Coker and Shiladitya DasSarma
Evaluated 5 Oct 2009

Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.
Walker LM, Bowley DR, Burton DR
J Mol Biol 2009 Jun 5 389(2):365-75 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Valerie Huse and Shiladitya DasSarma
Evaluated 1 Sep 2009

Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role.
Saelensminde G, Halskau Ø, Jonassen I
Extremophiles 2009 Jan 13(1):11-20 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Melinda Capes and Shiladitya DasSarma
Evaluated 9 Feb 2009

Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.
Williams RS, Moncalian G, …, Russell P, Tainer JA
Cell 2008 Oct 3 135(1):97-109 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Priya DasSarma and Shiladitya DasSarma
Evaluated 10 Dec 2008

DNA is preserved and maintains transforming potential after contact with brines of the deep anoxic hypersaline lakes of the Eastern Mediterranean Sea.
Borin S, Crotti E, …, Corselli C, Daffonchio D
Saline Systems 2008 Aug 5 4(1):10 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 13 Aug 2008

Predictive behavior within microbial genetic networks.
Tagkopoulos I, Liu YC, Tavazoie S
Science 2008 Jun 6 320(5881):1313-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Anna-Louise Reysenbach / Philip Benfey / James A Coker and Shiladitya DasSarma / Hunter Fraser
Evaluated 6 Aug 2008

Use of the DiversiLab repetitive sequence-based PCR system for genotyping and identification of Archaea.
Cleland D, Krader P, Emerson D
J Microbiol Methods 2008 Feb 25 [abstract on PubMed] [related articles] [full text]
Selected by | Shiladitya DasSarma
Evaluated 4 Apr 2008

Neutron scattering reveals extremely slow cell water in a Dead Sea organism.
Tehei M, Franzetti B, …, Ginzburg M, Ginzburg BZ
Proc Natl Acad Sci U S A 2007 Jan 16 104(3):766-71 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 13 Feb 2007

Analysis of tryptic digests indicates regions of GvpC that bind to gas vesicles of Anabaena flos-aquae.
Dunton PG, Mawby WJ, Shaw VA, Walsby AE
Microbiology 2006 Jun 152(Pt 6):1661-9 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 3 Jan 2007

Reassembly of shattered chromosomes in Deinococcus radiodurans.
Zahradka K, Slade D, …, Lindner AB, Radman M
Nature 2006 Oct 5 443(7111):569-73 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 20 Oct 2006

Cyanobacteria toxins in the Salton Sea.
Carmichael WW, Li R
Saline Systems 2006 2:5 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 23 Jun 2006

Mutations and rearrangements in the genome of Sulfolobus solfataricus P2.
Redder P, Garrett RA
J Bacteriol 2006 Jun 188(12):4198-206 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Donald Ward / Shiladitya DasSarma
Evaluated 22 Jun 2006

Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei.
Britton KL, Baker PJ, …, Esclapez J, Rice DW
Proc Natl Acad Sci U S A 2006 Mar 28 103(13):4846-51 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 18 Apr 2006

Proteorhodopsin lateral gene transfer between marine planktonic Bacteria and Archaea.
Frigaard NU, Martinez A, Mincer TJ, DeLong EF
Nature 2006 Feb 16 439(7078):847-50 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma / Tamar Barkay
Evaluated 28 Feb 2006

Increased salinization of fresh water in the northeastern United States.
Kaushal SS, Groffman PM, …, Band LE, Fisher GT
Proc Natl Acad Sci U S A 2005 Sep 20 102(38):13517-20 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 24 Jan 2006

An expanding family of archaeal transcriptional activators.
Ouhammouch M, Geiduschek EP
Proc Natl Acad Sci U S A 2005 Oct 25 102(43):15423-8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 1 Dec 2005

DNA damage detection by an archaeal single-stranded DNA-binding protein.
Cubeddu L, White MF
J Mol Biol 2005 Oct 28 353(3):507-16 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma
Evaluated 28 Oct 2005

Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm).
Echigo A, Hino M, …, Kamekura M, Usami R
Saline Systems 2005 1:8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Oct 2005

Heat shock response of Archaeoglobus fulgidus.
Rohlin L, Trent JD, …, Gunsalus RP, Liao JC
J Bacteriol 2005 Sep 187(17):6046-57 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 14 Sep 2005

Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands.
Guy CP, Bolt EL
Nucleic Acids Res 2005 33(11):3678-90 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Jul 2005

The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication.
Henneke G, Flament D, …, Querellou J, Raffin JP
J Mol Biol 2005 Jul 1 350(1):53-64 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma
Evaluated 15 Jun 2005

Leptosphaeria rhodopsin: bacteriorhodopsin-like proton pump from a eukaryote.
Waschuk SA, Bezerra AG, Shi L, Brown LS
Proc Natl Acad Sci U S A 2005 May 10 102(19):6879-83 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 6 May 2005

Life at depth: Photobacterium profundum genome sequence and expression analysis.
Vezzi A, Campanaro S, …, Bartlett DH, Valle G
Science 2005 Mar 4 307(5714):1459-61 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 16 Mar 2005

pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12.
Maurer LM, Yohannes E, …, Radmacher M, Slonczewski JL
J Bacteriol 2005 Jan 187(1):304-19 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Jan 2005

Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis.
Hendrickson EL, Kaul R, …, Olson MV, Leigh JA
J Bacteriol 2004 Oct 186(20):6956-69 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 18 Nov 2004

Cultivation of Walsby's square haloarchaeon.
Burns DG, Camakaris HM, Janssen PH, Dyall-Smith ML
FEMS Microbiol Lett 2004 Sep 15 238(2):469-73 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma
Evaluated 27 Oct 2004

Microarray analysis of transposition targets in Escherichia coli: the impact of transcription.
Manna D, Breier AM, Higgins NP
Proc Natl Acad Sci U S A 2004 Jun 29 101(26):9780-5 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 19 Jul 2004

Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons.
Acinas SG, Marcelino LA, Klepac-Ceraj V, Polz MF
J Bacteriol 2004 May 186(9):2629-35 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma / Camilla Nesbo
Evaluated 24 May 2004

Environmental genome shotgun sequencing of the Sargasso Sea.
Venter JC, Remington K, …, Rogers YH, Smith HO
Science 2004 Apr 2 304(5667):66-74 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma / Rino Rappuoli / Douglas Capone / Gerard Muyzer / Ian M Head
Evaluated 9 Mar 2004

Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption.
Schelert J, Dixit V, …, Drozda M, Blum P
J Bacteriol 2004 Jan 186(2):427-37 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 25 Feb 2004

Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus.
Robinson NP, Dionne I, …, Bernander R, Bell SD
Cell 2004 Jan 9 116(1):25-38 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 19 Jan 2004

Chaperonins govern growth of Escherichia coli at low temperatures.
Ferrer M, Chernikova TN, …, Golyshin PN, Timmis KN
Nat Biotechnol 2003 Nov 21(11):1266-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma / Ramon Serrano
Evaluated 8 Dec 2003

The COG database: an updated version includes eukaryotes.
Tatusov RL, Fedorova ND, …, Yin JJ, Natale DA
BMC Bioinformatics 2003 Sep 11 4:41 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 15 Oct 2003

Mechanisms of thermal adaptation revealed from the genomes of the Antarctic Archaea Methanogenium frigidum and Methanococcoides burtonii.
Saunders NF, Thomas T, …, Land M, Cavicchioli R
Genome Res 2003 Jul 13(7):1580-8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 31 Jul 2003

Repair of UV damage in Halobacterium salinarum.
McCready S, Marcello L
Biochem Soc Trans 2003 Jun 31(Pt 3):694-8 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Shiladitya DasSarma
Evaluated 25 Jun 2003

Diversification and spectral tuning in marine proteorhodopsins.
Man D, Wang W, …, Spudich JL, Béjà O
EMBO J 2003 Apr 15 22(8):1725-31 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 13 May 2003

Archaea and their potential role in human disease.
Eckburg PB, Lepp PW, Relman DA
Infect Immun 2003 Feb 71(2):591-6 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Feb 2003

A microarray-based antibiotic screen identifies a regulatory role for supercoiling in the osmotic stress response of Escherichia coli.
Cheung KJ, Badarinarayana V, …, Janse D, Church GM
Genome Res 2003 Feb 13(2):206-15 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Feb 2003

Coordinate regulation of energy transduction modules in Halobacterium sp. analyzed by a global systems approach.
Baliga NS, Pan M, …, Ng WV, Hood L
Proc Natl Acad Sci U S A 2002 Nov 12 99(23):14913-8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 8 Jan 2003

A novel archaeal transcriptional regulator of heat shock response.
Vierke G, Engelmann A, Hebbeln C, Thomm M
J Biol Chem 2003 Jan 3 278(1):18-26 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 28 Nov 2002

The structures of four macrolide antibiotics bound to the large ribosomal subunit.
Hansen JL, Ippolito JA, …, Moore PB, Steitz TA
Mol Cell 2002 Jul 10(1):117-28 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 7 Oct 2002

Overexpression of a Na+/H+ antiporter confers salt tolerance on a freshwater cyanobacterium, making it capable of growth in sea water.
Waditee R, Hibino T, …, Incharoensakdi A, Takabe T
Proc Natl Acad Sci U S A 2002 Mar 19 99(6):4109-14 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 31 Jul 2002

The genome of M. acetivorans reveals extensive metabolic and physiological diversity.
Galagan JE, Nusbaum C, …, Metcalf WW, Birren B
Genome Res 2002 Apr 12(4):532-42 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma
Evaluated 29 May 2002

SHOT: a web server for the construction of genome phylogenies.
Korbel JO, Snel B, Huynen MA, Bork P
Trends Genet 2002 Mar 18(3):158-62 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 2 Apr 2002

Genomics and early cellular evolution. The origin of the DNA world.
Forterre P
C R Acad Sci III 2001 Dec 324(12):1067-76 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 8 Feb 2002

Genome trees constructed using five different approaches suggest new major bacterial clades.
Wolf YI, Rogozin IB, …, Tatusov RL, Koonin EV
BMC Evol Biol 2001 Oct 20 1:8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Shiladitya DasSarma / William Martin
Evaluated 11 Jan 2002

A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions.
López-García P, Moreira D, López-López A, Rodríguez-Valera F
Environ Microbiol 2001 Jan 3(1):72-8 [abstract on PubMed] [related articles] [order article]
Selected by | Shiladitya DasSarma
Evaluated 30 Nov 2001

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