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F1000 Factor 3.0

New Finding
Tech Advance
 An Atlas of Combinatorial Transcriptional Regulation in Mouse and Man.
Ravasi T, Suzuki H, …, Ideker T, Hayashizaki Y
Cell 2010 Mar 5 140(5):744-752 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Andrew D Sharrocks
Evaluated 18 Mar 2010

Exceptional
F1000 Factor 9.0

Tech Advance
 Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites.
Schilling O, Overall CM
Nat Biotechnol 2008 Jun 26(6):685-94 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Morten Meldal
Evaluated 18 Mar 2010

Recommended
F1000 Factor 3.0

New Finding
 Structure-guided design of potent and selective pyrimidylpyrrole inhibitors of extracellular signal-regulated kinase (ERK) using conformational control.
Aronov AM, Tang Q, …, ter Haar E, Xie X
J Med Chem 2009 Oct 22 52(20):6362-8 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Xiaohua Huang and Gerald Shipps
Evaluated 18 Mar 2010

Recommended
F1000 Factor 3.0

Tech Advance
 In-gel protein phosphatase assay using fluorogenic substrates.
Kameshita I, Baba H, Umeda Y, Sueyoshi N
Anal Biochem 2010 May 1 400(1):118-122 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Amy Barrios
Evaluated 17 Mar 2010

Recommended
F1000 Factor 3.0

New Finding
 Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli.
Anjem A, Varghese S, Imlay JA
Mol Microbiol 2009 May 72(4):844-58 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Chris Kristich and John Kirby
Evaluated 17 Mar 2010

Recommended
F1000 Factor 3.0

Tech Advance
 Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.
Muona M, Aranko AS, Raulinaitis V, Iwaï H
Nat Protoc 2010 Mar 5(3):574-87 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Mario Lebendiker
Evaluated 17 Mar 2010

Must Read
F1000 Factor 6.4

Confirmation
Hypothesis
New Finding
 The structure of the peripheral stalk of Thermus thermophilus H+-ATPase/synthase.
Lee LK, Stewart AG, …, Bernal RA, Stock D
Nat Struct Mol Biol 2010 Mar 17(3):373-8 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Stephan Wilkens / Michael Galperin
First evaluation 12 Mar 2010 | Latest evaluation 15 Mar 2010

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F1000 Factor 3.0

Tech Advance
 Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.
Agresti JJ, Antipov E, …, Griffiths AD, Weitz DA
Proc Natl Acad Sci U S A 2010 Mar 2 107(9):4004-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Petr Capek and Michal Lebl
Evaluated 12 Mar 2010

Must Read
F1000 Factor 6.0

Hypothesis
New Finding
Tech Advance
 The transition state for folding of an outer membrane protein.
Huysmans GH, Baldwin SA, Brockwell DJ, Radford SE
Proc Natl Acad Sci U S A 2010 Mar 2 107(9):4099-104 [abstract on PubMed] [related articles] [FREE full text]
Selected by | James Bowie
Evaluated 10 Mar 2010

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F1000 Factor 3.2

Hypothesis
New Finding
Controversial
 Ubiquitination acutely regulates presynaptic neurotransmitter release in Mammalian neurons.
Rinetti GV, Schweizer FE
J Neurosci 2010 Mar 3 30(9):3157-66 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Jane Sullivan / Noam Ziv
First evaluation 10 Mar 2010 | Latest evaluation 11 Mar 2010


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